Automated Construction of an Open Reading Frame Library from Sinorhizobium meliloti
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چکیده
منابع مشابه
Directed construction and analysis of a Sinorhizobium meliloti pSymA deletion mutant library.
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A plasmid was constructed that facilitates the cloning and expression of open reading frame DNA. A DNA fragment containing a bacterial promoter and the amino terminus of the cI gene of bacteriophage lambda was fused to an amino-terminally deleted version of the lacZ gene. An appropriate cloning site was inserted between these two fragments such that a frameshift mutation was introduced upstream...
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The endmost chromosome I ORF is silenced by a natural telomere position effect. YAR073W/IMD1 was found to be transcribed at much higher levels in sir3 mutants and when its adjacent telomere was removed from it. These results suggest that telomeres play a role in silencing actual genes.
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ژورنال
عنوان ژورنال: JALA: Journal of the Association for Laboratory Automation
سال: 2003
ISSN: 1535-5535
DOI: 10.1016/s1535-5535-04-00270-9